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HomeNanotechnologySingle molecule localization-based evaluation of clathrin-coated pit and caveolar dynamics

Single molecule localization-based evaluation of clathrin-coated pit and caveolar dynamics


Clathrin-coated pits and caveolae are nanoscale invaginations of the plasma membrane of cells, forming by way of the meeting of membrane coat and accent proteins in a tightly regulated course of. We’ve analyzed the event of those membrane coat constructions with excessive spatial and temporal decision and sensitivity utilizing super-resolution single-molecule localization microscopy (SMLM) on dwell cells. To this finish, we developed a complicated clustering and information evaluation workflow that robotically extracts the related data from SMLM picture sequences taken on dwell cells. We quantified lifetime distributions of clathrin-coated and caveolar constructions, and analyzed their progress dynamics. Furthermore, we noticed hotspots within the plasma membrane the place coat constructions seem repeatedly. The stunningly related temporal improvement of clathrin-coated and caveolar constructions means that key accent proteins, a few of that are shared between the 2 varieties of membrane coat constructions, orchestrate the temporal evolution of those complicated architectures.

Graphical abstract: Single molecule localization-based analysis of clathrin-coated pit and caveolar dynamics

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